摘要
针对花生基因组DNA常规提取方法的不足,对提取程序进行改良.改良后的方法具有简便、快速、安全、得率高等特点,对所鉴定植株生长影响相对较小,所提取DNA可直接用于PCR、限制性酶切等分子生物学试验.
A modified method to extract genome DNA from peanut was developed. The method can be used to carry out PCR and enzyme shearing reaction directly, and has many other advantages such as simpleness, rapidity, safety and high production and so on. Moreover, there is little harmful effect on plant growth when peanut genome DNA is extracted with this method.
出处
《福建农林大学学报(自然科学版)》
CSCD
北大核心
2003年第3期273-275,共3页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省科技厅资助项目(2002I021).