小鼠尿中沙美特罗及其代谢物的LC/MS研究

Determination of Salmeterol and Its Metabolites in Rat Urine by LC/MS

采用较大剂量小鼠灌胃给药 ,得到的尿样经固相提取后用 LC/ APCI/ MS分析 ,根据代谢知识及比较空白尿和阳性尿的响应数据 ,得出了沙美特罗的原型和四种代谢产物。结果表明 :小鼠尿中沙美特罗可能的主要代谢途径为羟基化、甲氧基化以及两者的加合。

15 mg of salmeterol is administrated to 10 rats, which weighs about 25 g each. The urine of the rats is extracted by solid-phase column and analyzed by liquid chromatography-APCI-mass spectrometry. Parent drug and four metabolites are identified by comparing total ion chromatograms of the blank with the metabolic urine as well as their mass spectra. Its main metabolic pathways are the hydroxylation and/or methoxylation from the experiments.