转移抑制基因nm23-H1在结直肠癌中的表达及其与临床病理因素的关系

Expression of metastasis suppressor gene nm23-H1 in colorectal carcinoma and its relationship with clinical pathological parameters

目的 探讨人结直肠癌组织中nm23-H1表达及其与临床病理因素的关系。方法 应用逆转录PCR及免疫组织化学(免疫组化)方法,分别测定30例结直肠癌手术切除标本及癌旁2、5cm组织和远端正常对照组织的nm23-H1 mRNA以及蛋白表达水平,分析nm23-H1与结直肠癌临床病理因素的关系。结果 结直肠癌中nm23-H1 mRNA的低表达与淋巴结转移及Dukes分期明显相关(P<0.05)。nm23-H1蛋白表达水平与结直肠癌中淋巴结转移、分化程度、Dukes分期呈明显的负相关(P<0.05),并同基因转录水平一致。结论 nm23-H1的降表达是结直肠癌转移中的重要的分子生物学事件,检测结直肠癌中nm23-H1的表达情况对评估患者的预后可能具有意义。

Objective To study the expression of nm23-H1 in human colorectal carcinoma and its relationship with clinical pathological parameters. Methods RT-PCR and immunohistochemical assay were used to determine the mRNA transcription level of nm23-H1 gene and its protein expression in primary tumors(T), para-tumor tissues 2 cm(P2) and 5 cm(P5) distant from primary tumor margin and distal normal tissues(N) in 30 patients with colorcctal carcinoma. The relationships between nm23-H1 expression and clinicopathological parameters were analyzed. Results The lower expression of nm23-H1 mRNA in colorectal carcinoma was correlated with lymph node metastasis and advanced Dukes' stage(P<0.05). There was significant negative correlation between nm23-H1 protein expression and clinicopathological parameters such as lymph node metastasis, tumor differentiation and advanced Dukes' stage(P<0.05). nm23-H1 gene transcription was coincident with its protein expression. Conclusions The decreased expression of nm23-H1 is an important molecular biological event during metastasis of colorectal carcinoma. The assay of nm23 H1 gene expression in colorectal carcinoma may be helpful to evaluate the prognosis of the patients with colorectal cancer.