摘要
目的 观察维生素 D衍生物 EB10 89对人胰腺癌细胞系 Bx PC- 3的生长抑制作用及其可能机制。方法 应用 MTT比色法、流式细胞术、Western blot法免疫印记电泳进行检测。结果 EB10 89抑制人胰腺癌细胞系 Bx PC- 3的生长 ,抑制 5 0 %细胞生长的药物浓度 (IC50 )为 10 - 7mmol/ L。癌细胞在EB10 89作用下 ,G0 / G1 期细胞比例增加 2 3% ,S期细胞比例下降 12 %。胰腺癌细胞系 Bx PC- 3的 p2 1蛋白的表达随 EB10 89的作用时间和药物浓度的提高而增强。结论 EB10 89对胰腺癌细胞系 Bx PC- 3具有生长抑制作用 ,其作用机制可能与 p2 1表达上调有关。
Objective To observe and investigate inhibitory effects of EB1089 on the growth of BxPC-3 human pancreatic cancer cell line BxPC-3. Methods The antiproliferative effects of EB1089 on BxPC-3 were detected by MTT test. The changes of cell cycle were investigated flow cytometry by using. The levels of p21 expression were measured by Western blotting.Result EB1089 inhibited proliferation of the BxPC-3 cells. The proportion of cells in the G 0/G 1 phase increased and combined with the decrease of proportion of cells in the S phase. Conclusions The results suggest that EB1089 inhibits the growth of certain pancreatic cancer cells by up regulation of p21, which is therefore considered to be a potentially useful agent for new therapeutic strategies focusing on inhibition of pancreatic cancer cell proliferation.
出处
《胰腺病学》
2003年第3期136-139,共4页
Chinese JOurnal of Pancreatology
