SLE与RA患者抗钙调素自身抗体的测定

Determination of Anti-Calmodulin Autoantibody in the Patients with Systemic Lupus Erythematosus and Rheumatoid Arthritis

用Phenyl-Sepharose 4B亲和色谱法自人脑提取CaM,以兔抗CaM为阳性对照,建立了检测抗CaM自身抗体的ELISA法。正常人(n=100)抗CaM活性为7.56±2.68U/ml,一般内科病人为8.45±5.25U/ml。而32例SLE和28例RA患者分别为26.15±11.69U/ml和13.61±9.66U/ml,阳性率分别为53.31％和21.43％,均显著高于正常对照。抗CaM自身抗体与抗核抗体的出现有较高的同步性(x^2=5.003,P<0.05)。

The activity of anti-Calmodulin (CaM) autoantibody in the sera was determined by an ELISA technique in which CaM purified from human brain was used as coating antigen and rabbit anti-CaM antibody as the positive control. The anti-CaM activities in normal controls (n = 100) , other cases (n = 50) , systemic lupus erythema-tosus (SLE) (n=32) and rheumatoid arthritis (RA) (n = 28) were 7.56±2.68U/ml ,8.45± 5.25U/ml, 26.15±11.69U/ml and 13.61±9.66U/ml respectively. Both the anti-CaM activities and anti-CaM positive rates in SLE and RA patients (53.31% and 21.43%) were significantly higher than in normal controls. There was a significant correlation be tween the presence of anti-CaM antibody and anti-nuclear antibody.