Poly(A)信号及3’非转译区对IL-2Rα表达调控的影响

Induction on the Expression and Regulation of Human IL-2Rα cDNA by Poly (A) Signal and 3'UTR

将IL-2Ra cDNA3’端系列缺失克隆的重组质粒导入CHO细胞,经DNA、RNA及蛋白质印迹分析表明:缺3’非转译区(3’UTR)及Poly(A)信号序列的缺失子DNA,可以整合到受体细胞染色体上,但不能形成稳定的mRNA及表达rIL-2Ra;IL-2Ra cDNA上1297bp处的ATTAAA序列独立地显示出Poly(A)信号功能,相应的缺失子DNA能转录形成稳定的mRNA和表达rIL-2Ra。结果表明:poly(A)尾是位于3’端的转译调控因子,3’UTR与IL-2Ra cDNA的表达调控密切相关。

A series of mutant deleted with Bal 31 nuclease in IL-2Rα cDNA 3' untranslation region (3'UTR) was introduced into CHOdhfr cells. Respectively ,bloting analysis of DNA,RNA and protein prepared from the CHOdhfr^+ clones suggested that 1)the deleted DNA without 3'UTR and Poly(A)signal only could integrate on the chromosomes of host cell ,but form into stable mRNA and produce IL-2Rα;2)the 'AT-TAAA' sited at 1297bp of IL-2Rα cDNA was of Poly (A) signal function alone, and the deleted DNA with 'ATTAAA' could transcribe into stable mRNA and translate into IL-2Rα efficiently. The results indicate that Poly(A) is a translation regulator at 3' end and 3'UTR is closely related to the regulation of gene expre-sion.