外周有关递质对疑核(AMB)调节细胞免疫机能的影响

The Effect of Peripheral Neurotransmitter on Nucleus Ambiguus (AMB) Regulating Cellular Immunity in Rabbits

本实验研究了三种受体拮抗剂对电刺激疑核调节细胞免疫机能的影响,并对疑核调节机体细胞免疫的机制作了探讨。实验用麻醉家兔,分3组进行。分别在静注阿托品、酚妥拉明或心得安后电刺激疑核,并分别于注射前及刺激后不同时间取外周血,测定T细胞百分率及对PHA的反应。结果:阿托品组上述免疫指标比注射前明显降低;酚妥拉明组上述免疫指标在刺激后60分钟时比注射前有所升高;心得安组上述指标在刺激后120分钟时比注射前明显降低。提示:疑核对细胞免疫机能的调节可能是通过迷走神经末梢释放乙酰胆碱,作用于免疫器官及免疫细胞上的M型胆碱能受体实现的。

In the present paper,the effect of periperal neurotransmitter on Nucleus Ambiguus (AMB) regulating cellular immunity were studied in rabbits. The AMB of animals were stimulated after Atropine or Phentolamine or Propranolol were injected from venous,and peripheral blood samples were taken before injection and 10,30,60,120 and 180 min after stimulation respectively to determine percentage of T cell and mito-genic response to phytohemagglutinin (PHA).The results wereiThe percentage of T cell and mitogenic response to PHA of Atropine group were decreased significantly at 10 min after stimulation untill 180 min after stimulation compared with that of before injection ;The percentage of T cell and mitogenic response to PHA of Phentolamine group were increased at 60 min to 180 min after stimulation compared with before injection; The percentage of T cell and mitogenic response to PHA of Propranolol group were decreased significantly at 120 min and 180 min after stimulation compared with before injection. The results suggested that:The AMB took part in the modulation of cellular immunity by vagus nerve and Ach is a neurotransmitter in the pathway.