脑梗死大鼠康复训练后脑功能恢复及病理学改变

Rehabilitative effects and pathological changes in rats after cerebral infarction

目的探讨康复对急性脑梗死大鼠脑功能恢复的作用机制及其病理学变化。方法采用抽签法将 3 0只Wistar大鼠随机分为 3组 :A组 (假手术组 )、B组 (造模组 )、C组 (康复组 )。运用血管内线栓法制备脑梗死动物模型 ,术后 2 4h、3d及 7d分别进行Bederson神经功能评分 ,平衡木、转棒、网屏测评 ,并于术后第 7d观察脑组织病理学变化。结果与A组比较 ,B、C组Bederson神经功能评分各时间点均有高度显著性差异 (P <0 0 1) ;各组大鼠术后 2 4h均出现抓握、行走及协调能力障碍 ,随着时间的延长 ,A组的各项功能很快恢复 ,B、C组功能改善远不及A组 ,有高度显著性差异 (P <0 0 1) ;与B组比较 ,缺血后 3d ,C组平衡木试验有显著性差异 (P <0 0 5 ) ;缺血后 7d ,除Berderson评分外 ,均有显著性差异 (P <0 0 5或P <0 0 1)。脑缺血第 7d ,与A组比较 ,B组大鼠缺血区脑组织明显水肿 ,周围区神经细胞数目明显减少 ;与B组比较 ,C组大鼠脑缺血区脑组织水肿减轻 ,神经细胞数目增多。结论康复能提高脑梗死大鼠平衡、行走及抓握能力 ;减轻大鼠脑缺血区脑组织水肿 ,增加周围区神经细胞数目。

ObjectiveTo study the rehabilitative effects and pathological changes in rats after acute cerebral infarction. Methods 30 male Wister rats were randomly divided into 3 groups :Group A(sham group),Group B(model group) and Group C(rehabilitation group). Animal model was made by the middle cerebral artery occlusion(MCAO).For each group, Bederson Neural Function was scored and balancing wood,rotating bar,and net screen were tested at 24 hours,3 days and 7 days after operation respectively. On the 7th day after operation, pathological change of brain tissue was observed. ResultsCompared with Group A, Bederson Neural Function scores of Group B,C indicated significant differences at each time (P<0.01). The abilities of grasping, walking and coordinating of all the groups after 24 hours by operation handicapped, however with time continuing,every function of Group A restored and that of Group B or C improved partly,but showed significant difference compared with Group A(P<0.01). After 3 days by operation,balancing wood test of Group C was better than that of group B(P<0.05), after 7 days,except Bederson Neural Function score,there were difference between Group C and B(P<0.05 or P<0.01). After 7 days by operation,compared with Group A,brain tissue of infarction area in Group B indicated significantly edema, neural cells decreased. Compared with Group B, the edema in Group C decreased and neural cells increased.ConclusionsRehabilitation therapy can improve the MCAO rats' ability of equilibrating, grasping and walking, and decrease edema, increase neural cells in infarction areas. [