抗除草剂转基因油菜向杂草诸葛菜的潜在基因漂移

The Potential Herbicide-resistant Gene Flow of Transgenic Glyphosate-resistant and Glufosinate-resistant Oilseed Rapes to Orychophragmus Violaceus

以抗除草剂草甘膦和草丁膦转基因油菜为父本 ,诸葛菜为母本 ,在人工授粉条件下研究抗性基因向诸葛菜的潜在基因漂移。采用荧光显微镜观察转基因油菜花粉在诸葛菜柱头上的萌发生长情况 ,并与诸葛菜自花授粉的情况进行比较。结果显示 2种转基因油菜花粉在诸葛菜柱头上的萌发生长情况类似。花粉粘合的数量比自交时显著减少 ,出现粘合延迟现象 ;花粉在柱头上不能正常萌发生长 ,有的呈现明显的畸形 ,花粉管不能穿过乳突细胞 ,更不能进入花柱和胚囊发生受精 ,这些结果表明两者的亲和性较差。授转基因油菜的花粉后诸葛菜不能结实也进一步证明了两者的亲和性较差。上述结果表明抗除草剂转基因油菜的抗性基因向诸葛菜漂移的可能性较小。

The potential herbicide-resistant gene flow of glyphosate-resistant and glufosinate-resistant transgenic rape to Orychophragmus violaceus were studied under pollination. The germination and growth of male pollen grain on female stigmas were observed with fluorescence microscope. The results showed pollen grain germination and growth of two varieties of transgenic oilseed rapes on the female stigmas was similar. Compared with self-pollination of O. violaceus, the adhesion number of pollen grains decreased significantly and adhesion line delayed; pollen grains couldn't germinated normally with abnormal pollen tubes. Pollen couldn't penetrate papilla cell, enter styles and embryo sacs. The observing results demonstrated the sexual compatibility between them was very low. The fact that emasculated O. violaceus pollinated with the males pollen grains could not germinate, further proved the incompatibility between them. All results showed the potential gene flow between them were very low.