摘要
目的 获得mICoS-Ig基因修饰的小鼠DC,分析表达产物mICOS-Ig对T细胞活化的影响。方法 采用电转染方法将mICOS-Ig表达载体转入小鼠DC,通过ELISA法检测转染小鼠DC 60和96 h以及稳定表达的培养上清;观察mICOS-Ig对同种混合细胞培养反应的影响。结果 ELISA法检测证明将mICOS-Ig表达载体转入小鼠DC,获得瞬时表达及稳定表达,mICOS-Ig可抑制单向混合淋巴细胞反应,抑制率达40％,其效应呈剂量依赖性。结论 获得了稳定表达mICOS-Ig融合蛋白的小鼠DC,mICOS-Ig表达产物对同种细胞刺激的增殖反应有抑制作用。
Objective To obtain mouse dendritic cells transfected with mICOS-Ig gene and evaluate effects of mICOS-Ig gene products on T cell activating function.Methods mICOS-Ig expression vector was transferred into mouse dendritic cells by electroporation. ELISA was used to detect the supernatant of mouse dendritic cells at the time of 60 and 96 h after transfection and stable expression, the effects of mICOS-Ig on homologous mixed lymphocyte culture was observed using Balb/c and C57BL/10J mouse as reacting cells and stimulating cells respectively. Results We obtained transient and stable expression after mICOS-Ig expression vector transfected into mouse dendritic cells, mICOS-Ig can inhibit single mixed lymphocyte culture reaction. Conclusion We obtained stable expression after ICOS-Ig expression vector transfected into mouse dendritic cells, and could be further used in other study.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2003年第5期339-341,共3页
Immunological Journal
基金
国家"973"计划资助项目(CB510008)
