B7修饰K562细胞来源的树突状细胞抗肿瘤作用的研究

Study on the anti-tumor effect of DC derived from K562 transfected with B7 gene

目的 探讨共刺激信号分子B7转化人白血病细胞株K562后,K562-B7在细胞因子作用下分化为树突状细胞(Dendritic cell,DC),K562-B7-DC的生物学特性以及介导的抗肿瘤免疫作用。方法 构建重组真核表达质粒pcDNA3.1D7转化人白血病细胞株K562,获得单个细胞克隆。用K562-B7细胞株在GM-CSF、IL-4和IL-12的联合作用下分化为DC细胞,观察K562-B7-DC的生物学性质及其抗肿瘤的免疫功能。结果 K562-B7-DC在GM-CSF、IL-4和IL-12的联合作用下能分化为DC细胞并能诱导出较强的同种混合淋巴细胞反应及CTL活性,且能分泌内源性IL-12、INF-γ。结论 细胞因子联合诱生的K562-B7-DC能有效的执行其抗原提呈功能,同时协同T淋巴细胞发挥其抗肿瘤免疫作用。

Objective To assess the biological alteration and anti-tumor immune effect of the DCs derived from K562 transfected with B7 gene. Methods B7 gene was inserted into eukaryotic expression plasmid pcDNA3.1B7, the K562 with plasmid pcDNA3.1B7 was transfixed, then positive clone(K562-B7) was selected. The K562-B7-DCs was obtained by inducement with GM-CSF, IL-4 and IL-12. Then,the biological nature and the effect of anti-tumor including allo-MLR, CTL and secretionof IL-12, INF-γ were analyzed. Results The K562-B7-DCs can be induced by GM-CSF,IL-4 and IL-12, and have the potential ability to stimulate allo-MLR, CTL and secretion of IL-12, INF-γ. Conclusion K562-B7-DCs induced by GM-CSF, IL-4 and IL-12 have the function of antigen presenting cells, and can help T lymphocyte to execute the anti-tumor effect, therefore enhancing anti-tumor immunity.