PPAR-γ的激动剂抑制肺癌细胞生长的实验研究

Experimental research about peroxisome proliferator-activated receptor-γ activator inhibiting human lung cancer cell growth

目的 研究肺癌细胞上过氧化物酶体增生物激活受体γ(PPAR γ)的表达及其经配体 (激动剂 )活化后抑制肺癌细胞生长的机制。方法 以RT PCR和Westernblot检测肺癌细胞上PPAR γ的表达 ,并通过MTT和细胞计数检测经PPAR γ的激动剂作用后的细胞增殖情况 ,以TUNEL检测细胞的凋亡情况。结果 两种细胞上均有PPAR γ的表达 ;PPAR γ的配体作用后明显抑制细胞生长 ,且与时间和剂量有关 ;经配体活化的PPAR γ能诱导细胞凋亡 ,使其增殖受抑。结论 作为肺癌治疗的新靶点———PPAR γ在肺癌细胞上表达 ,且经配体活化后能通过诱导凋亡而抑制肺癌细胞的生长 。

AIM To study the expression peroxisome proliferator activated receptor γ(PPAR γ) in lung cancer cell and the mechnism of PPAR γ activated by ligands inhibiting human lung cancer cell growth.METHODS PPAR γ was detected in two lung cancer cell lines by RT PCR and Western blot, the growth inhibition of human lung cancer cell was investigated by MTT and cell counts,and the apoptosis was assessed by TUNEL. RESULTS PPAR γ express in two lung cancer cell lines,which can inhibit human lung cancer cell growth after being activated. PPAR γ agonists can induce apoptosis of lung cancer cell. CONCLUSION As a new target for lung cancer therapy, PPAR γ express in lung cancer cells and can inhibit their growth through induction of apoptosis.