摘要
目的 探讨D 半乳糖对小鼠骨代谢的影响及其可能机制。方法 3mon龄昆明种♀小鼠每日颈背部皮下注射D 半乳糖 10 0 0mg·kg-1·d-1,连续 4 2d ,取右侧股骨测定骨干重、骨羟脯氨酸、骨钙、骨微量元素 ,同时检测抗氧化酶活性及脂质过氧化产物。结果 D 半乳糖可使骨干重、骨羟脯氨酸、骨钙减少 ,骨干重与体重比值 ,骨镁与骨干重比值降低 ;同时 ,抗氧化酶活性下降 ,脂质过氧化产物增加。结论 D 半乳糖可导致 3mon龄小鼠骨质丢失 。
AIM To investigate the effects of D galactose on bone contents of hydroxyproline(HOP), calcium, microelements and activities of antioxidation in mice. METHODS Twenty female kunming mice at three months of age were used in this study. D galactose at dose of 1 g·kg -1 ·d -1 was given subcutaneous injection daily to the mice for 42 days. The right femurs were collected to determine the bone dry weight, bone hydroxyproline content, bone calcium, and bone microelements. The activities of catalase (CAT), glutathione peroxidase (GSH Px) and superozide dismutases (SOD) in blood, and contents of methylenedioxyamphetamine (MDA) in serum, lipofuscin in liver were determined. RESULTS The bone dry weight, hydroxyproline, calcium of bone decreased significantly in D galactose treaded group(compared with control group, P <0 01). The body weight was also reduced. However, the decrease of bone hydroxyproline and calcium is greater than that of body weight. The index of bone dry weight/body weight and bone magnesium/bone dry weight decreased significantly( P <0 01 and P <0 05). The activities of CAT, GSH PX, SOD in blood were depressed( P <0 05) and contents of MDA in serum, lipofuscin in liver increased( P <0 05).CONCLUSION D galactose at the age of the proper dose can induce bone loss in mice at three months. The mechanism may due to decrease of bone magnesium and declining of activation of antioxidation.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2003年第9期1017-1019,共3页
Chinese Pharmacological Bulletin
基金
广东省科技计划重点项目资助课题 NoA3 0 10 3 0 2 0 1
关键词
半乳糖
骨代谢
骨羟脯氨酸
骨微量元素
galactose
bone metabolism
bone hydroxyproline
bone magnesium
