异种心脏移植急性血管排斥反应期动物模型的建立

Establishment of the Model for Acute Vascular Rejection in Heart Xenotransplantation

为建立异种心脏移植急性血管排斥反应期动物模型,选择豚鼠和SD大鼠分别作为供体和受体。豚鼠升主动脉与大鼠腹主动脉端侧吻合,豚鼠主肺动脉与大鼠下腔静脉端侧吻合,受体鼠术前静脉注射眼睛蛇毒因子(CVF)60U/kg,术后腹腔注射CVF60U·kg-1·8h-1,抑制超急性排斥反应,另腹腔注射细胞免疫排斥反应抑制剂(FK506)0.25mg·kg-1·6h-1。结果显示:移植成功率88.9%(16/18),平均存活时间24.00±3.05h,病理学检查发现排斥心脏内广泛血栓形成,间质出血及炎症细胞浸润,部分心肌有局灶梗塞和凝固性坏死,符合急性血管排斥反应期的表现。该模型手术操作简单,不需显微镜,稳定可靠,是研究异种心脏移植急性血管排斥反应期较理想的动物模型。

To establish the model of acute vascular rejection in heart xenotransplantation, the Guinea pigs and SD rats were served as donors and recipients respectively. Heart from the donor was transplanted heterotopically into the recipient, the aorta of the donor heart was anastomosed endtoside to the abdominal aorta of the recipient and the pulmonary artery was anastomosed endtoside to the inferior vena cava. To prevent hyperacute rejection, Cobra venom factor (CVF) was given intravenously at a dosage of 60U·kg-1 preoperatively , same dosage was given by an i.p. injection per 8 hours postoperatively. To prevent cellular rejection, FK506 was given at a dosage of 0.25mg·kg-1·6h-1 by an i.p. injection per 6 hours postoperatively. 18 operations of transplantation were done with the successful rate of 88.9%, the mean survival time of the xenografts was 24±3.05 hours. The histopathology of the rejected xenografts showed diffuse intravascular thrombosis, interstitial hemorrhage associated with a large number of infiltrated inflammatory cells, focal ischemic infarcts and coagulative necrosis, consisted with the histological description of the acute vascular rejection reported by others. We could draw the conclusion that the guinea pigtorat heart transplantation model could be performed without microscope, its simplicity, easiness and reliability suggest that it is a good model in studying acute vascular rejection in heart xenotransplantation.