生育及不育男性血清及精浆抑制素-B水平分析

Inhibin B Levels of Serum and Seminal Plasma in Fertile and Infertile Males

目的 :探讨生育及不育男性血清及精浆抑制素 B(inhibinB ,INHB)水平是否存在差异 ,了解血清及精浆INHB水平与精子发生的关系。 方法 :生育组 (n =2 0 )、少精子症组 (n =2 0 )、弱精子症组 (n =2 2 )和非阻塞性无精子症 (NOA)组 (n =2 0 )男性于上午 8∶0 0～ 10∶0 0留取精液和血液标本 ,进行精液常规分析 ,血清INHB、FSH、LH、T含量 ,精浆INHB、酸性磷酸酶、果糖、α 葡糖苷酶含量和活性测定。 结果 :血清、精浆INHB水平与血FSH均呈显著负相关 (r =- 0 .5 36 ,P <0 .0 0 1vsr =- 0 .2 88,P =0 .0 1) ,血清、精浆INHB水平与精子密度均呈显著正相关 (r=0 .49,P <0 .0 0 1vsr =0 .48,P <0 .0 0 1) ,血清INHB水平在生育组男性与少精子症组、NOA组男性间(分别为P <0 .0 5和P <0 .0 1)、弱精子症组与NOA组男性间 (P <0 .0 1)及少精子症组与NOA组男性间 (P <0 .0 5 )差异均有显著性 ,而精浆INHB变动范围较大 ,其水平仅在生育组男性与NOA组男性间及弱精子症组与NOA组男性间差异有显著性 (分别为P <0 .0 1和P <0 .0 5 )。精浆INHB水平与精浆α 葡糖苷酶活性呈正相关 (r=0 .377,P =0 .0 0 1)。血清INHB水平与精浆INHB水平间无相关性。 结论 :血清、精浆INHB水平均可反映睾丸的精子发生情况 。

Objectives: To investigate the possible differences in the inhibin B levels of seminal plasma and serum between fertile and infertile males and to obtain information on the relation between serum inhibin B or seminal plasma inhibin B and spermatogenesis. Methods: Semen and blood samples were collected from fertile(n=20), oligospermia(n=20), asthenospermia(n=22)and non-obstructive azoospermia(NOA)(n=20) males at 8∶00 am～10∶00 am. Semen parameters were analyzed. Levels of inhibin B in seminal plasma and serum, ACP, Fru, α-Glu in seminal plasma, serum levels of FSH, T, LH were determined. Results: Both levels of serum inhibin B and levels of seminal plasma inhibin B correlated significantly negatively with serum FSH(r=- 0.536, P< 0.001 vs r=- 0.288, P= 0.01), and statistically positively with sperm concentration(r= 0.49, P< 0.001 vs r= 0.48, P< 0.001).There was positive correlation between levels of seminal plasma inhibin B and activity of α-Glu in seminal plasma (r= 0.377, P= 0.001). The difference in levels of seminal plasma inhibin B was found only between fertile males or asthenospermia and NOA(P< 0.01 and P< 0.05, respectively). However, significant differences in levels of serum inhibin B were found not only between males with normal sperm concentration (including fertile males and asthenospermia) and NOA (P< 0.01), fertile males and oligospermia (P< 0.05), but also between oligospermia and NOA (P< 0.05).There was no correlation between serum inhibin B and seminal plasma inhibin B.Conclusions: Both levels of serum inhibin B and seminal plasma inhibin B could reflect testis spermatogenesis status. Levels of seminal plasma inhibin B could also reflect the function of seminiferous duct, but the wide range of values limited its applicability.