大黄鱼体表溃烂症病原菌的鉴定

Classification for one pathogen isolated from skin-ulcer Pseudosciaena crocea

从网箱养殖的患体表溃烂症的大黄鱼心、肝、肾、脾等组织分离到8株细菌,大黄鱼人工感染试验表明分离菌株XS01为致病菌。对该菌株进行形态、生理生化试验鉴定为费氏弧菌(Vibriofischeri)。药敏试验结果表明,该菌株对磺胺甲基异恶唑等13种药物敏感,对氨苄青霉素等8种药物不敏感。进行了该菌株对温度和盐度的耐受性试验,认为该菌有较广的温度和盐度适应范围。

Eight strains were isolated from the skinulcer disease of Pseudosciaena crocea in 2000 in Xiangshan Area of Zhejiang Province. Strain XS01 was confirmed to be the pathogen of the disease by artificial infection test. According to the results of morphological features, physiological and biochemical characteristics that were very similar to those of Vibrio fischeri, the XS01 were identified as Vibrio fischeri (V.fischeri). Meanwhile, its sensitivity to 25 chemotherapeutants was detected. The results showed that ampicillinum(AMP) and other 7 kinds of antibiotic had no inhibitory activity to strain XS01, and it was sentsitive to sulfamethoxazolum(SLZ) and other 12 kinds of antibiotic . Finally, based on the results obtained, strain XS01 has broad expanse of tolerance of salt and temperature.