干酪素法测定大黄鞣质类成分的研究

Content Determination of Tannins in Rheum officinale by Casein Method

目的 :建立稳定的干酪素法测定大黄鞣质的方法。方法 :用干酪素法测定大黄鞣质。优化条件为 :以 3 0 %甲醇回流提取 3 0min ,干酪素用量 60 0mg ,1.5 %Na2 CO3 显色后 15min测定。结果 :优化方法线性关系良好 ,鞣酸平均回收率为99.2 7% ,RSD =1.0 0 % (n =3 )。结论 :该法简单、灵敏、稳定 。

Objective:To establish a stable method for determining the content of tannins in Rheum officinale by casein method.Methods:The casein method was used to determine the content of tannins.The optimum conditions of determination were using 30% methyl alcohol as extracting solvent, refluxing 30 min, adopting 600 mg caseins, adding 1.5% Na 2CO 3 and determining after 15 min.Results:The selected method had a good linear dependence. The average recovery of tannic acid was 99.27 % with RSD =1.00%( n =3).Conclusion:It is simple, sensitive and reproducible to determine the content of tannins in Rheum officinale .