摘要
目的 研究三甲氧苄嗪 ( 10 -6 M)对离体心脏低温保存的影响 ,并探讨其作用机制。方法 以改良的Langendorff离体鼠心灌注模型 ,观察三甲氧苄嗪加入UW(三甲氧苄嗪 - 10 -6 M)液后 ,离体鼠心在 4℃保存 6h后血流动力学、冠脉流出液心肌酶漏出量、心肌含水量、心肌ATP、MDA含量、心肌超微结构的变化。结果 UW液加入三甲氧苄嗪组与未加入三甲氧苄嗪组相比 ,复灌后心功能恢复率明显增高 ( p<0 .0 1) ;心肌含水量 ( p<0 .0 5 )和丙二醛含量较低 ( p<0 .0 1) ;心肌酶漏出量明显减少 ( p<0 .0 1) ;心肌ATP水平得到很好地维持 ( p <0 .0 1) ;心肌超微结构尤其是线粒体结构改变轻 ,损伤小。结论 10 -6
OBJECTIVE Evaluate the effect of 10 -6Mtrimetazidine on rat hearts during cold storage. METHODS With Langendorff perfused isolated rat heart , we added trimetazidine to UW solution till final concentration of 10 -6M. After preserved in the UW solution with or without trimetazidine at 4℃ for 6 hours ,the following items were observed in the hearts , the changes of hemodynamics , myocardial enzyme leakage in the coronary effluent , myocardial tissue ATP content , MDA content , myocardial water content and the ultrastructural changes of myocardium . RESULTS Compared with the myocardium in UW solution , the hearts in UW solution with containing 10 -6M trimetazidine had higher recovery rate of cardiac function and myocardial tissue ATP content(p<0.01), lower myocardial water content(p<0.05), myocardial tissue MDA content and myocardial enzyme leakage(p<0.01), slighter ultrastructural change of myocardium , especially the mitochondrial damage . CONCLUSION Trimetazidine (10 -6M) could protect the rat hearts in low temperature pressevation.
出处
《中国体外循环杂志》
2003年第2期88-91,共4页
Chinese Journal of Extracorporeal Circulation
基金
2 0 0 3年教育部优秀青年教师资助计划项目
