兔胚胎关节软骨细胞体外培养的研究

The behaviour or rabbit fetal articular chondrocytes cultured in vitro

目的 探讨兔胚胎软骨细胞体外培养的生物学特性。方法 对孕4周兔胚胎关节软骨用酶消化法分离培养细胞,观察细胞存活率、贴壁率、生长曲线和组织形态学改变。结果 兔胚胎软骨细胞可从胚胎软骨组织中消化分离出来,经鉴定具有软骨细胞的特性。原代兔胚软骨细胞存活率达 97％以上,细胞贴壁率达 80％以上,从原代到第 4代都有高增殖力,到第 8代时增殖力降低,到第12代时几乎丧失细胞增殖。结论 体外培养的胚胎软骨细胞前4代适合于作修复关节软骨缺损的组织工程细胞。

Objective To investigate the biological characteristics of the behaviour of rabbit fetal articular chondrocytes cultured in vitro. Methods Fetal 4-week-old rabbit articular chondrocytes were isolated and cultured to observe cell survival rate, cell adhesive rate, growth curves and morphological changes. Results Embryonic chondrocytes were isolated from embryonic articular cartilage tissues and proved to be chondrocytes. The cell survival rate and cell adhesive rate of embryonic chondrocytes in pri- mary culture were more than 97% and 80% respectively. The growth curves showed that the prolifera- tion of the primary to the fouth passage was powerful, and to the 8th passage, decreased apparently, and to the 12th lort th proliferative ability. Conclusion The methods of isolation, culture and identification of rabbit embryonic articular chondrocytes were presented and discussed. On the basis of the embryonic chondrocytic proliferation the early passage embryonic chondrocytes (around the 4th passage) may be su- perior tothe primary in the cartilage tissue engineering.