Evaluation of proliferative activities in Wilms′ tumor

目的 :评价 PCNA标记指数及 Ag NORs计数在肾母细胞瘤增殖活性判定中的意义。方法 :对 34例肾母细胞瘤标本进行 PCNA免疫组织化学染色及核仁组织区嗜银蛋白组织化学染色。结果 :PCNA- LI与病理学分型及临床分期无关 ,但 PCNA- LI≥ 2 5 %组的 S期细胞数、PI值及Ag NORs数量均显著高于 PCNA- LI<2 5 %组 ;Ag NORs计数与病理学分型及临床分期关系密切 ;PCNA- LI与 Ag NORs计数结合可准确反映肾母细胞瘤的增殖活性。结论 :目前肾母细胞瘤的病理学分型和临床分期可以反映其侵袭性 ,但并不充分 ;在准确评价肾母细胞瘤生物学特性方面 ,PCNA- LI与 Ag

Objective: To assess the potential significance of proliferating cell nuclear antigen (PCNA) labelling indexes (PCNA LI) and AgNORs number in evaluation of proliferative activity of Wilms′ tumor. Methods: A silver staining for AgNORs and an immunohistochemical method PCNA staining were performed on the biopsy specimens taken from 34 children with Wilms′ tumor. Results: No significant differences were observed between PCNA LI and the pathological types and clinical stages, whereas fraction of S phase and PI and the number of AgNORs were significantly higher in patients with PCNA LI≥25% than that in patients with PCNA LI<25%. The number of AgNORs/cell correlated well with both pathological types and clinical stages. The combination of PCNA LI and AgNORs can accurately reflect the proliferative activity of cancer cells in Wilms′ tumor. Conclusion: The current pathological types and clinical stages may reflect the aggressive activity in Wilms′ tumor, but insufficient. The simultaneous determination of PCNA LI and AgNORs count could be used as the essential complementarity of conventional pathological types and stages for accurate evaluation of biologic behaviour of Wilms′ tumor.