细胞色素P450Ⅱ E1-基因多态性在酒精性肝病发病中的意义

The Significance of Cytochrome P450ⅡE1 Genetic Polymorphism of Pathogenesis in Alcoholic Liver Disease

目的 :探讨细胞色素 P45 0 E1的基因型与酒精性肝病的相关性。方法 :采用 PCR- RFLP分析方法对 1 0 0例酒精性肝病患者 ,1 5 0例非酒精性肝病患者 ,2 5例酗酒无肝病者和 5 0例正常对照者进行 CYP45 0 E1多态性分析。结果 :CYP45 0 El分成 A型为 C1基因野生纯和子 ,B型为( C1 /C2 )基因的杂合子 ,C型为 C2基因的突变型纯合子。酒精性肝病中 A、B、C型分别 3%、93%、4%;酗酒无肝病组 A型为 1 0 0 %;非酒精性肝病中 A、B型分别 74%、2 6%;健康志愿者 A、B型分别 72 %、2 8%。酒精性肝病与酗酒无肝病组、非酒精性肝病及健康志愿者比较 ,有统计学意义。乙肝引起肝癌与酒精相关性肝癌的 A型明显减少 ,而 B型则明显增多 ,有统计学意义。结论

Objective: To explore the relationship between genotype of cytochrome P450ⅡE1 and alcoholic liver disease. Methods:PCR RFLP method was used. Results:According to CYP450 Ⅱ E1 gene, type A was the hemozygote of wild type in gene C1,type B was the heterozygote of gene (C1/C2) and type C was the mutational hemozygote of gene C2 .Type A, B and C in the group of alcoholic liver disease were 3%, 93% and 4%, respectively. The rate of type A in the group of drinking without liver disease was 100%. Type A and B in the group of non alcoholic liver disease were 74% and 26%. And that in normal control groups were 72% and 28%. Comparing alcoholic liver disease group with drinking without liver disease group and comparing the patients with non alcoholic liver disease with the normal control group, the results were siginificant in statistics. Comparing liver carcinoma resulting from hepatitis B with alcohol, type A was more lower and B was more higher obviously. Conclusion: Gene C2 plays a role in the association between pathogenesis of alcoholic liver disease and occurrence of alcoholic liver carcinoma.