摘要
目的 探讨氟对体外培养的大鼠破骨细胞抗酒石酸酸性磷酸酶 (TRAP)和基质金属蛋白酶 9(MMP 9)活性的影响。方法 通过机械分离大鼠乳鼠四肢长骨的方法培养破骨细胞 ,应用组织化学方法观察氟对培养的破骨细胞TRAP活性的影响 ;应用原位杂交方法观察氟对培养的破骨细胞MMP 9mRNA表达的影响。结果 染氟组单位面积的TRAP阳性细胞表达增加 ,各剂量组TRAP阳性细胞数分别为 15 4 2、16 0 0、170 6、179 0和 180 0个 /cm2 ,但差异无显著性 ;随着染氟剂量的增加 ,MMP 9mRNA的表达增加 ,以 1 0 0、2 0 0和 4 0 0mg/L染氟组最为明显 ,依次为 94 5 0、94 6 4和10 4 97,与对照组比较差异有显著性。结论 氟对破骨细胞MMP 9mRNA的表达有增强作用。
Objective To investigate the effects of fluoride on activities of tartrate-resistant acid phosphate (TRAP) and matrix metallproteinase-9 (MMP-9) in rat osteoclasts cultured in vitro . Methods Osteoclast was isolated mechanically from long bones of neonatal rats and cultured in vitro . Histochemical stain was applied to detect the effects of fluoride on activities of TRAP and in-situ hybridization was used to study the expression of MMP-9 mRNA in rat osteoclasts in vitro . Results Number of TRAP positive cells was 154.2, 160.0, 170.6, 179.0 and 180.0 per cm 2, respectively for the rats with varied doses of fluoride, in a dose-response pattern but without statistical significance. The expression of MMP-9 mRNA increased with elevating dose of fluoride, especially in the rats with 1.00, 2.00 and 4.00 mg/L of fluoride, to 94.50, 94.64 and 104.97, respectively, significantly different from those in control group. Conclusions Fluoride can enhance the MMP-9 mRNA expression in cultured osteoclasts of rats.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2003年第4期256-258,F003,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金重点资助项目 (3 973 0 3 90 )
