有活性的人类同种带瓣管道的制备和保存

Preparation and Preservation of Viable Human Homograft Valve Conduits

目的 :建立长期有效的有活性的人类同种带瓣管道的制备及保存方法。方法:无菌条件下取健康成年、脑死亡6h以内志愿损献者的心脏 ,修剪成同种带瓣主、肺动脉管道 ,37℃灭菌12h ,程控降温至 -60℃ ,取出后放入液氮罐中保存。应用光镜、电镜及流式细胞仪比较新鲜和液氮保存后的同种带瓣管道在组织结构、超微结构及细胞活性之间的差异。结果 :程控降温后液氮保存的同种带瓣管道与新鲜标本在组织结构、超微结构及细胞活性等方面无明显区别。结论 :无菌条件下制备的同种带瓣管道经程控降温、液氮保存后结构完整 ,具有良好的细胞活性 。

Objective:To establish long-term valid methods of preparation and preservation of viable human homograft valve conduits(HVC).Methods:The healthy hearts were removed under sterile condition from adult volunteers who died within6hours,and were trimed into homograft aortic and pulmonary valve conduit.After12h sterilization,they were cooled to-60degrees through controlled rate.All HVC were cryopreserved in liquied nitrogen.The fresh and croypreserved HVC in tissue structure and cell viability were compared by means of optical microscope,electron microscope,and Flow Cytometry.Results:There was no alteration of cell viability and form of cryopreserved HVC.Conclusion:The sterile cryopreserved HVC can keep the intact tissue structure and favorable cell viability,and can be used as a kind of heterogeneous material.