美洛宁对大鼠重型颅脑创伤后海马区神经细胞凋亡及记忆功能的影响

The Effect of CTP on Neuronal Apoptosis and Cognitive Function after Serve Traumatic BrainInjury in Rats

目的 :研究美洛宁对大鼠重型颅脑创伤后海马区神经细胞凋亡及学习记忆功能的影响。方法 :采用Marmarou的方法建立大鼠重型闭合性颅脑创伤模型 ,将成年Wistar大鼠288只随机分为脑创伤组、美洛宁治疗组、假手术组 ,每组又分别分为伤后3 ,6,12 ,24 ,48 ,72、168及336h等8个时相组 ,另取12只作为正常对照组。美洛宁治疗组经致伤后 ,给予腹腔注射美洛宁 (30mg·kg-1·d -1) ,直至各时相点处死 ;脑创伤组、假手术组及正常对照组在相同时间给予等量的生理盐水腹腔注射作为对照。各组均在相应的时间点处死取材进行原位细胞凋亡检测。另取48只大鼠随机分为脑创伤组、美洛宁治疗组、假手术组及正常对照组 ,进行水迷宫测试。结果 :美洛宁能够使海马区神经细胞凋亡的高峰明显下调 ,使水迷宫测试的潜伏期明显缩短。结论 :美洛宁能够有效的抑制大鼠重型颅脑创伤后海马区神经细胞凋亡 。

Objective:To investigate the effect of CTP on neuronal apoptosis and cognitive function after severe traumatic brain injury(TBI)in rats.Methods:The model of severe closed TBI was established according to the method created by Marmarou.Two hundred and twenty-eight Wistar rats were randomly divided into TBI group,CTP treating group,and fake operation group,and each of them was divided into3,6,12,24,48,72,168,and336hour groups.Twelve rats were taken as normal control.The CTP treating group after injury was treated with CTP[30mg/(kg·d)]in peritoneum.TBI group,the fake operation group,and control group were treated with Saline.All rats were killed at each time point,and apoptosis in situ were detected.Another48rats were randomly divided into brain injury group,CTP treating group,fake operation group,and normal group.The cognitive dysfunction was evaluated using the Morris water maze(MWM).Results:CTP could decrease the apex of apoptosis-positive cells in hippocampus and shorten the delitescence of Morris water maze test.Conclusions:CTP may effectively suppress apoptosis of nerve cells after TBI and improve cognitive function.