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骨髓间充质干细胞体外分化过程中端粒酶活性表达及意义 被引量:1

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摘要 目的 :探讨体外诱导兔骨髓间充质干细胞分化为内皮细胞及其扩增过程中端粒酶活性的表达及其意义。方法 :联合应用密度梯度离心和贴壁培养法分离骨髓间充质干细胞 ,继而诱导其向内皮细胞方向分化并传代培养。用TRAPezeELISA法和TRAP银染法分别检测骨髓间充质干细胞体外诱导分化前后端粒酶活性。结果 :兔骨髓间充质干细胞体外培养时具有快速增殖能力 ,在特定环境中诱导分化细胞具有内皮细胞特征 ;增殖前的骨髓间充质干细胞端粒酶活性低水平表达 ,一旦经过诱导分化其端粒酶活性表达上调 ,在 5代内其端粒酶活性不因细胞传代而下降或消失。结论 :骨髓间充质干细胞在体外有限扩增和诱导分化时保持端粒酶活性 ,维持组织干细胞特性 ,为内皮祖细胞的临床研究奠定理论基础。
出处 《实用医学杂志》 CAS 2003年第8期851-853,共3页 The Journal of Practical Medicine
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