荧光定量聚合酶链反应检测解脲支原体感染的临床意义

Fluorescence quantitative PCR detected infection of Ureaplasma urealyticum.

目的 采用荧光定量聚合酶链反应 (FQ -PCR)技术 ,准确定量检测待检标本中解脲支原体 (UU)的感染状况。方法 采用FQ -PCR技术 ,检测男女生殖道标本 76 5份 ,并同时用常规PCR技术 ,对比检测了 85例。结果 FQ -PCR检测76 5份标本 ,UU -DNA阳性 2 79份 ,阳性率 36 .5 % ,其DNA平均拷贝数为 2 .4× 10 4。 38例阳性患者治疗两周内复查 ,转阴率仅为 4 4 .7% ,治疗 3w后复查 ,转阴率达 76 % (P <0 .0 0 5 )。常规PCR结果重复检测时 ,2 5例阳性中 2例变为阴性 ,而 6 0例阴性中 4例变为阳性。FQ -PCR结果重复时完全吻合。结论 FQ -PCR检测UU ,具有快速、特异性高、定量准确等优点 ,并可为临床评价疗效提供依据。

Objective: Infection of Ureaplasma urealyticum( UU) was detected by real time FQ-PCR technology.Method:765 cases specimens of UU- DNA quantification from male and femal reproduc five tract were examined by automated of real time quantification with the ABI Prism 7700 Sequece Detector. Comparison detected 85 cases by FQ-PCR and ordinary PCR. Results: UU- DNA was found in 279 of 765(36.5%). DNA average amount of positive samples was 2.4×104. 38 cases second were examined in treatment two week, negative cases 17 of 38(44.7%), after treatment three week, negative cases 29 of 38(76%),P<0.005. 2 cases changed from positivity to negativity in 25 cases and 4 cases changed from negativity to positivity in 60 cases when results of ordinary PCR was repeated. Conclusion:Real time FQ-PCR is a good method for measuring UU load.