标本采集时间对精液全自动分析各参数的影响

Influence of the interval time of semen Acquisition to the parameters by computer assiofted sperm analysis.

目的 为了了解精液分析中标本获取时间间隔对结果的影响 ,我们对 4 4个健康受试者的精液进行了测试。方法 受试者 4 8h内留取精液两次 ,采用伟力全自动精子分析仪对精液进行全自动分析。分别对已婚、未婚 ,2 4h、4 8h结果进行统计学分析。结果 两次取精中第二次精液量明显减少 ,且差异具有统计学意义。精子运动速度参数vsl、vcl、vap ,在已婚者中差异具有不同程度的统计学意义。精子密度、a级精子率两次差异均无统计学意义 ,而b级精子率和c级精子率两次差异均具有不同程度的统计学意义。结论 进行精液常规分析中应要求 :取精间隔超过 4 8h ,并且最好每次检查前禁欲天数尽量保持一致 ,从而使检验结果具有可信和可比性。也有利于临床医生的诊断 ,及治疗效果的比较。

Objective: In order to study influence of the interval time of sperm acquisition to the parameters of the computer assisted sperm analysis(CASA), we detected 44 semens with healthy men. Method: Analysed the semens acquired twice within 48 hours using Wei-li CASA and did statistics study. Results: The quantity of semen in the second was less than that in the first and it was significant in statistics.The difference of velocity parameters about sperm motility (vsl?vcl?vap) between two cases in the married men was significant in statistics. The differences of sperm concentration and A grade percent were not significant in statistics between two cases. The differences of B grade and C grade percent were significant in statistics between two cases. Conclusion: When we do routine semen analysis, the interval time of semen acquisition should be more than 48 hours and the time of abstinence should be conformable before the patient acquires semen to analysis every time.These make the outcome creditability and comparability.These help doctors diagnosis and treatment.