胰岛素样生长因子-1受体在人胰腺癌细胞中的表达和意义

INSULIN-LIKE GROWTH FACTOR-1 RECEPTOR EXPRESSION AND SIGNIFICANCE IN HUMAN PANCREATIC CANCER CELLS

目的 探讨胰腺癌细胞 (PC- 3)胰岛素样生长因子 - 1受体 (IGF- 1R)基因的定量表达 ,及其与细胞凋亡、成瘤性的关系。方法 用逆转录 PCR方法 ,检测胰岛素样生长因子 - 1受体 m RNA的表达量 (灰度比值 ) ,以 β2 微球蛋白基因作为内参照 ,以 K5 6 2细胞作为阳性对照。体外采用凋亡、软琼脂集落形成 ,体内采用裸鼠成瘤性的方法观察经 IGF- 1R反义寡核苷酸(ASON)有效降低 IGF- 1R m RNA后的变化。结果 PC- 3细胞 IGF- 1R基因表达量高于阳性对照组 K5 6 2 ,PC- 3细胞 IGF- 1R的 m RNA相对表达量为 1.2 5± 0 .11,K5 6 2细胞 IGF- 1R的 m RNA相对表达量为 1.14± 0 .0 9,两者的 IGF- 1R m RNA相对表达量差异无显著意义 (P>0 .0 5 )。 IGF- 1Rm RNA的表达量降低后 ,PC- 3细胞失去了增殖能力 ,14 d后仍无集落形成 ,裸鼠移植瘤明显变小。结论 PC- 3细胞的 IGF- 1R基因过度表达 ,经 IGF- 1R ASON有效降低其基因表达量后 ,PC- 3细胞失去了增殖能力。

Objective To explore Insulin like growth factor 1 receptor(IGF 1R)gene quantitative expression in human pancreatic cancer cells(PC 3) and the relationship of IGF 1R with apoptosis?tumorigenicity.Methods The expression of IGF 1R gene in PC 3 cells was quantitatively determined by reverse transcript polymerase chain reaction(RT PCR) combined with β 2 micro globlin(β 2GM) gene as inner control to amplificate synchronously,and with K562 as positive control.The grey value ratio of them were determined.apoptosis and soft agar colongenicity in vitro,and their tumorigenicity in nude mice in vivo for the transfected IGF 1R ASON cells were observed.Results The expression of IGF 1R in PC 3 cells was high than in the positive control K562(P>0.05).The PC 3 cells transfected IGF 1R ASON lost proliferative capacity,no colonogenicity after 14 days,and the tumor become smaller in nude mice.Conclusion The gene of IGF 1R gene was overexpression in PC 3 cells,and the proliferative capacity was lost after transfected IGF 1R ASON.