海藻酸钠-氯化钡微囊对大鼠胰岛体外胰岛素分泌功能的影响

THE EFFECT OF ALGINATE-BACL_2 MICROCAPSULES ON THE INSULIN SECRETORY FUNCTION OF RAT ISLETS IN VITRO

目的 观察海藻酸钠 -氯化钡微囊对大鼠胰岛体外胰岛素分泌功能有无影响。方法 以海藻酸钠和氯化钡为材料 ,采用气体吹喷制囊法将新鲜分离纯化的大鼠胰岛制成微囊化胰岛 ,取空微囊、微囊化大鼠胰岛与未微囊化大鼠胰岛各 5 0 0只 ,分为 10份 ,置于培养板中培养 ,用放免法测定并比较第 2、4、6 d培养液中基础胰岛素浓度。结果 空微囊组第 2、4、6 d的基础胰岛素平均浓度均为 0 nm ol/ 1/ 5 0 ,微囊化大鼠胰岛组第 2、4、6 d的基础胰岛素平均浓度为 5 .179、5 .80 6、5 .5 5 8nm ol/ 1/5 0只 ,未微囊化大鼠胰岛组第 2、4、6 d的基础胰岛素平均浓度为 5 .4 4 1、6 .0 80、5 .4 6 8nmol/ 1/ 5 0只 ,后两者差异无显著性意义 (P>0 .0 5 )。结论 海藻酸钠

Objective To observe the effect of Alginate-BaCl 2 microsapsules on the insulin secretory function of rat islets in vitro.Methods We took alginate,Barium Chloride and rat islets which were isolated and purified from rat pancreas freshly as materials,and used air-jet method to make microencapsulated islets.500 hollow microcapsules,500 microencapsulated islets and 500 non-microencapsulated islets were cultured in culture plate respectively,50 islets per well.The culture media were changed every 2 nd day.To measure the basal insulin concentration in the culture medium at 2 nd ,4 th ,6 th day by radioimmunassay method and compare the results.Result The mean insulin concentration in the media of the hollow microcapsule group at 2 nd ,4 th ,6 th day were 0 nmol/1/50,the mean insulin concentration in the media of microencapsulated islet group were 5.179,5.806,5.558 nmol/1/50,and that of non-microencapsulated islet group were 5.441?6.080?5.468nmol/1/50.There were no significant differences betwee the microencapsulated islet group and non microencapsulated islet group(P>0.05).Conclusions Alginate-BaCl 2 microsapsules have not effect on the insulin secretory function or rat islets in vitro.