FasL在大鼠隐睾中的表达

Expression of FasL in Rat Cryptorchidism

目的 :观察FasL蛋白在大鼠隐睾中的表达并探讨其意义。 方法 :将 2 2日龄SD雄性大鼠 2 4只随机分为隐睾组和假手术组各 1 2只 ,在日龄 1 1 0d时采血并处死。免疫组化 (SP法 )测睾丸组织中的FasL蛋白表达 ,ELISA法检测血清抗精子抗体 (AsAb)。 结果 :隐睾组大鼠的隐睾侧及对侧睾丸的FasL阳性表达率均显著高于假手术组大鼠的手术侧及对侧睾丸 (P <0 .0 0 1 ) ;隐睾组和假手术组的血清AsAb阳性率分别为 41 .7%和 0 ,差异有显著性 (P <0 .0 1 )。 结论 :单侧隐睾大鼠的双侧睾丸组织中都有FasL表达的上调 。

Objective: To investigate the expression of FasL in rat cryptorchidism and its significance. Methods: Twenty four male SD rats (22 day old) were randomly divided into two groups: unilateral cryptorchid group ( n =12) and pseudo operation group ( n =12).When the rats were 110 day old, blood samples were taken and the rats were killed for analysis. Immunohistochemical method(SP) was used to detect FasL expression in testes and ELISA method to detect serum antisperm antibody (AsAb). Results: The prositive FasL expression rates in cryptorchid and contralateral testes were significantly higher than those in pseudo operation group ( P < 0.001 ). The serum AsAb positive rates in the cryptorchid group and the pseudo operation group were 41.7 % and 0, respectively, with significant difference( P < 0.01 ). Conclusions: FasL expression upregulating in both testes of the unilateral cryporchid rat may be a protective response of the testis to autoimmunity.