利用cDNA微阵列技术筛选成年期与胚胎期睾丸差异表达基因——钙联接蛋白基因

Molecular Cloning and Identification of Calmegin Gene in Embryo and Adult Testes by cDNA Microarray Hybridization

目的 :用cDNA微阵列技术克隆和筛选成年期与胚胎期睾丸差异表达基因 ,以进行睾丸发育及精子发生的调控研究。 方法 :构建人睾丸cDNA芯片 ,分别用正常成人及胚胎睾丸的mRNA探针进行杂交 ,对成人和胚胎睾丸中差异表达的基因进行高流量的比较。 结果 :发现 1条成人睾丸高表达、胚胎低表达的基因———钙联接蛋白 (CLGN)基因。 结论

Objective: To identify gene differential expression in embryo and adult testes by cDNA microarray techniques. Methods: cDNA probes of embryo and adult testes were used to hybridize the cDNA microarray of human testis, and the clones of differential hybridization were sequenced and analyzed. Results: The calmegin ( CLGN ) gene which had been confirmed to be involved in spermatogenesis was found, and it exhibited more than 20 fold difference at expression level between adult and embryo human testes. Conclusion: Genes differential expression in adult and embryo human testes can be identified by cDNA microarray hybridization.