川芎嗪对家兔阴茎海绵体平滑肌细胞游离钙浓度的影响

Effects of Telramethylpyraze on Cytosolic Free Calcium Concentration in Penis Corpus Cavernsum Smooth Muscle Cells in Rabbits

目的 :研究中药川芎嗪 (TMP)对体外培养家兔阴茎海绵体平滑肌细胞 (PCSMC)胞质内游离钙浓度([Ca2 + ] i)的影响。 方法 :用新型Ca2 + 荧光染色剂Fluo 3/AM负载家兔PCSMC ,细胞分为氯化钾 (KCl)作用组和去甲肾上腺素 (NE)作用组 ,应用激光扫描共聚焦显微镜 (LSCM)实时测定胞质内 [Ca2 + ] i的变化 ,分别观察不同浓度的TMP对高钾和NE诱导胞质内 [Ca2 + ] i升高的影响 ,并与经典钙拮抗剂维拉帕米 (Ver)的作用相对比。 结果 :静息状态下 ,TMP对家兔PCSMC胞质内 [Ca2 + ] i无明显影响。 1、1 0、1 0 0 μmol/LTMP能显著抑制高钾诱发的细胞内 [Ca2 + ] i升高 ,抑制率分别为 (38.6± 3 .0 ) %、(44.1± 2 .4) %和 (53 .7± 4 .1 ) % ;也能抑制 1 μmol/LNE诱发钙库释放所致的细胞内 [Ca2 + ] i升高 ,抑制率分别为 (1 3 .9± 2 .7) %、(2 1 .2± 1 .9) %和 (2 9.5± 3 .6) %。 结论 :TMP通过对家兔PCSMC电压依赖性钙通道和细胞内钙库释放的双重抑制作用 ,降低PCSMC胞质内 [Ca2 + ] i水平 ,其作用效果与Ver相似 ,这是TMP治疗阴茎勃起功能障碍的重要机制。

Objective: To study the effects of Chinese medicine telramethylpyraze (TMP) on intracellular free calcium (\[Ca 2+ \] i ) in cultured penis corpus cavernsum smooth muscle cell (PCSMC) in rabbits. Methods: By using laser scanning confocal microscope (LSCM), the \[Ca 2+ \] i fluorescence signal changes was investigated in cultured PCSMC loaded with Ca 2+ indicator Fluo 3/AM and divided into potassium chloride(KCl) group and norepinephrine (NE) group. Compared with verapamil (Ver), the effects of TMP was observed in different concentrations on \[Ca 2+ \] i increase induced by high potassium and NE. Results: TMP had no obvious effect on resting PCSMC \[Ca 2+ \] i . It was found that 1, 10, 100 μmol/L TMP significantly inhibited \[Ca 2+ \] i increase induced by high potassium depolarization. The peak inhibition rates were ( 38.6 ± 3.0 )%?( 44.1 ± 2.4 )% and ( 53.7 ± 4.1 )% respectively. TMP could also inhibit cytosolic calcium pool release induced by 1 μmol/L NE. The peak inhibition rates were ( 13.9 ± 2.7 )%, ( 21.2 ± 1.9 )% and ( 29.5 ± 3.6 )% respectively. Conclusions: TMP can inhibit rabbit PCSMC \[Ca 2+ \] i significantly by suppressing voltage dependent calcium channel and cytosolic calcium pool release. The effect, similar to Ver, signifies the important mechanism of erectile dysfunction (ED) therapy.