一氧化氮在新生鼠窒息后肾损伤中的作用研究

Research on effect of nitric oxide in post-asphyxial renal injury in neonatal rats.

目的 研究一氧化氮 (NO)在窒息后肾损伤中的作用。方法 将 4 5只 7- 10日龄Wistar大白鼠随机分为四组 ,即 :对照组 (13只 ) ,窒息后复氧 2h组 (10只 ) ,窒息后复氧 2 4h组 (11只 ) ,窒息后复氧 4 8h组 (11组 )。制成常压窒息模型。在上述各时点取左肾称重求出左肾系数 (LRC) ,并分别采用单一试剂法检测肾组织中NO的含量 ,同时在光镜下对肾小管损伤程度进行评分。结果 窒息后复氧 2h开始肾组织NO含量开始升高 ,在 2 4达高峰 ,同时左肾系数、肾小管评分也呈一致的改变。且NO的变化与左肾系数、肾小管评分均成显著正相关关系 (r分别为 0 .5 6和 0 .6 4 ;0 .78和 0 .6 7;0 .6 0和 0 .72 ;P均<0 .0 5 )。结论 NO参与了窒息后肾损伤的发生过程。

Objective: To research the effect of nitric oxide in post-asphyxial renal injury in neonatal rats. Methods: 45 neonatal Wistar rats of 7-10 days were randomly divided in to 4 groups: the control (n=13), 2h of post-asphyxia (n=10), 24h of post-asphyxia (n=11), and 48h of post-asphyxia (n=11). The experiment groups were established by normobaric asphyxia. In different times after asphyxia for 30 minutes, The left renal coefficient (LRC) was calculated. The content of nitric oxide (NO) of renal tissue were measured. And the sores of tubules were determined under light microscope. Results: Compared with the control, NO?LRC and the scores of tubules began to increased from 2h post-asphyxia and to reached the peak at 24h post-asphyxia. Moreover, the contents of NO in renal tissue were positively correlated with LRC and the scores of tubules. Conclusion: It is suggested that NO may play an important role in the development of post-asphyxia renal injury.