比较PCR酶免法与套式PCR法在不孕症中沙眼衣原体的临床检测

Comparison of PCR-ELISA and nested PCR for diagnosis of Chamydia trachomatis in sterility.

目的 比较PCR酶免法 (PCR -ELISA)与套式PCR法 (nPCR)法检测沙眼衣原体 (CT)在不孕症宫颈分泌物中的临床意义。方法 应用PCR -ELISA法对 185份宫颈分泌物进行CT检测 ,nPCR法对 112份宫颈分泌物进行CT检测 ,两种方法同时对 38份宫颈分泌物进行检测 ,两种方法的检测结果进行对比分析。结果 PCR -ELISA法 ,CT阳性率为 36 .2 2 % ,(6 7/ 185 )。nPCR法检测法CT阳性率为 19.6 4 % (2 2 / 112 )两种方法同时检测的CT阳性率分别为 34.2 1%及 13.16 %。PCR -ELISA与nPCR法比较差异有显著性 (P <0 .0 5 )。结论 PCR -ELISA法检测CT比nPCR法更敏感 ,特异 ,简单 ,并且没有EB污染等优点 ,结合临床治疗结果分析更适用于对长期慢性CT感染不孕症的诊断。

Objective: to evaluate the applicability of PCR-ELISA and nested PCR in detecting Chlamydia trachomatis (CT) in sterility. Method 185 and 112 cervical secretions of patients were identified by PCR-ELISA and nPCR respectively, while 38 cervical secretions of patients were collected for detecting CT by the two methods. Results 185 cervical specimens were identified by PCR-ELISA, the positive rate was 36.5%(67/185). 112 cervical specimens were identified by nPCR, the positive rate was 19.64%(22/112). In 38 cervical specimens, the positive rate was 34.21%(13/38) and 13.16%(5/38) by using PCR-ELISA and nPCR respectively, there was significance between the two methods (P<0.05). Conclusion: Compared with nPCR, PCR-ELISA is simple, sensitive, without EB pollution and suitable for clinical diagnosis of sterility of chronic CT infection.