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不同血清型马立克氏病病毒A抗原的比较研究 被引量:4

A COMPARATIVE STUDY ON ANTIGEN A OF MAREK'S DISEASE VIRUS OF DIFFERENT SEROTYPES
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摘要 收获马立克氏病病毒(MDV)3个不同血清型的细胞培养上清,用饱和硫酸铵法分别浓缩制备 A 抗原.经琼脂免疫扩散试验和 ELISA 抑制试验初步证明:MDV-GA 株(血清Ⅰ型)产生的 A 抗原 GA-A 与火鸡疱疹病毒 HVT-FCl26株(血清Ⅲ型)的 A 抗原 HVT-A 相似;而 MDV-SB-1株(血清Ⅱ型)的 A 抗原SB-1-A 与 GA-A 和 HVT-A 均不同.GA-A 于感染后第4天就能用琼扩检测到,并维持到第7天以后,而且产量较高;而 HVT-A 仅于感染后第5、6天呈现琼扩阳性,且滴度较低。由 MDV 强毒致弱的毒株 MDV-Mdll/75c 用琼扩未能检测到,可能已丧失了产生 A 抗原的能力。据试验结果可推测,不同毒株及其代次是影响 A 抗原产量的决定因素. Antigen A of Marek's disease virus(MDV)of different serotypes were concentrated 100 foldswith 50% saturated ammonium sulfate from the infected cell cuhure.Antigen A produced by MDV-GA(Serotype Ⅰ)and HVT-FC126(SerotypeⅢ)strains were shown the same in the antigenieity byimmunodiffusion and ELISA.However,antigen A produced by MDV-SB-1(SerotypeⅡ)differed fromthose of MDV-GA and HVT-FCI26.MDV-GA yielded more antigen A than strain HVT-FC126.AntigenA of MDV-GA could be detected in cell culture supernatant between the 4th to 7th day postinfeetion byimmunodiffusion.Antigen A of HVT-FC126 was detected only at 5th and 6th day postinfeetion.Incontrast,antigen A of MDV-Mdll/75c which was an attenuated strain from MDV-Mdll in tissue cul-ture,could not be detected in immunodiffusion.It was suggested that strain MDV-Mdll/75c,lost thecapacity of production of antigen A along with the loss of pathogenicity.It was concluded that the differentstrains and passages of MDV were deeisive factors affecting the yield of antigen A.
机构地区 兽医学系
出处 《南京农业大学学报》 CAS CSCD 北大核心 1992年第2期102-106,共5页 Journal of Nanjing Agricultural University
基金 高校博士点科技基金
关键词 马立克氏病 病毒 抗原 Marek's disease virus antigen A biological characterization immunodiffusion test ELISA inhibitation test
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