不同血清型马立克氏病病毒A抗原的比较研究

A COMPARATIVE STUDY ON ANTIGEN A OF MAREK'S DISEASE VIRUS OF DIFFERENT SEROTYPES

收获马立克氏病病毒(MDV)3个不同血清型的细胞培养上清,用饱和硫酸铵法分别浓缩制备 A 抗原.经琼脂免疫扩散试验和 ELISA 抑制试验初步证明:MDV-GA 株(血清Ⅰ型)产生的 A 抗原 GA-A 与火鸡疱疹病毒 HVT-FCl26株(血清Ⅲ型)的 A 抗原 HVT-A 相似;而 MDV-SB-1株(血清Ⅱ型)的 A 抗原SB-1-A 与 GA-A 和 HVT-A 均不同.GA-A 于感染后第4天就能用琼扩检测到,并维持到第7天以后,而且产量较高;而 HVT-A 仅于感染后第5、6天呈现琼扩阳性,且滴度较低。由 MDV 强毒致弱的毒株 MDV-Mdll/75c 用琼扩未能检测到,可能已丧失了产生 A 抗原的能力。据试验结果可推测,不同毒株及其代次是影响 A 抗原产量的决定因素.

Antigen A of Marek's disease virus(MDV)of different serotypes were concentrated 100 foldswith 50% saturated ammonium sulfate from the infected cell cuhure.Antigen A produced by MDV-GA(Serotype Ⅰ)and HVT-FC126(SerotypeⅢ)strains were shown the same in the antigenieity byimmunodiffusion and ELISA.However,antigen A produced by MDV-SB-1(SerotypeⅡ)differed fromthose of MDV-GA and HVT-FCI26.MDV-GA yielded more antigen A than strain HVT-FC126.AntigenA of MDV-GA could be detected in cell culture supernatant between the 4th to 7th day postinfeetion byimmunodiffusion.Antigen A of HVT-FC126 was detected only at 5th and 6th day postinfeetion.Incontrast,antigen A of MDV-Mdll/75c which was an attenuated strain from MDV-Mdll in tissue cul-ture,could not be detected in immunodiffusion.It was suggested that strain MDV-Mdll/75c,lost thecapacity of production of antigen A along with the loss of pathogenicity.It was concluded that the differentstrains and passages of MDV were deeisive factors affecting the yield of antigen A.