摘要
本试验研究了影响黑麦草悬浮细胞生长以及原生质体分离和培养的部分因素.结果表明:黑麦草悬浮细胞继代培养时的接种量对悬浮细胞的生长速度影响较大,其与培养基的比例为1∶20时,生长速度较快;当悬浮细胞与酶液的比例为2～3∶10时,每 ml 酶液原生质体产量最高;原生质体培养基中用葡萄糖代替蔗糖和甘露醇作碳源或渗透压稳定剂进时,原生质体的植板率较高。
The suspension growth,protoplast isolation and culture of Lolium mulliflorum Lam.andL.perenne-L.were studied.Rycgrass suspension cultures were in higher growth volamc subculturcd in thevolume ratio of 1 ml cells to 20 ml liquid medium.The enzyme solution produccd maximum yield of theprotoplasts when the proportion of cells in enzyme solution was 20%～30%.Glucose was more suitablethan sucrose and mannitol in protoplast medium as carbon source or osmoticum and led to higher platingefficiency of ryegrass protoplasts.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1992年第2期10-15,共6页
Journal of Nanjing Agricultural University
关键词
黑麦草
原生质体
碳源
rycgrass
protoplast
carbon source
osmoticum
