“911”抗HBV作用的实验研究

STUDY ON "911" ANTI-HBV EFFECT IN Hep G2 2215 CELLS CULTURE

目的 :评价药物“911”在 2 2 15细胞培养中对 HBs Ag和 HBe Ag表达的抑制作用。方法 :在 HBV转染的人肝癌 2 2 15细胞加入待检药物培养 ,收取细胞培养液 ,检测上清液中 HBs Ag和 HBe Ag浓度 ,细胞提取物作 HBV- DNA斑点分子杂交。结果 :“911”对 2 2 15细胞的毒性较小 ,TC50 为 4 4 .8m g/ml;对 2 2 15细胞的 HBe Ag有一定的抑制作用 ,且呈剂量反应关系 ,其抑制抗原半数有效浓度为 17.3m g/kg;治疗指数为 3.37。结论 :“911”对 2 2 15细胞内 HBV- DNA有一定的抑制作用 ,呈剂量反应关系 ,对 2 2 15细胞的 HBs Ag有较小抑制作用。

Objective:To evaluate the effect of '911' anti-HBV in Hep G 2 2215 cell culture.Methods: Different doses of '911' were added to the Hep G 2 2215 cell for culturing, supernatant was collected and HBsAg, HBeAg were detected.HBV-DNA in the extract of cell was detected by dot hybridization.Results:The toxicity was very low and TC 50 was 44.8mg/ml; '911' had certain extent of inhibit effect for HBeAg,there was a dose-effect relationship and the IC50 was 17.3mg/ml,TI was 3.37.Conclusion: '911' also can inhibit the HBV-DNA replication but only a small inhibited effect for HBsAg can be found.