自体成骨细胞—nBGC复合物修复犬胫骨骨缺损

Experiment on the Effect of nBGC Scaffold Loaded with Autologous Osteoblasts on the Repair of Canine Bone Defects/

目的:评价搭载自体骨细胞的纳米仿生材料在体内的组织反应性和骨修复作用。方法:4只犬的双侧胫骨各制作一直径10mm的单侧皮质骨缺损,一侧骨缺损植入复合自体成骨细胞的仿生生物材料作为实验组,另一侧植入自固化磷酸钙(CPC)作为对照组。于术后8周取材,观察各组X线片、组织学、四环素荧光标记变化,比较骨缺损修复情况。结果:X线检查显示nBGC支架与周围骨组织很快融合,界限模糊;而对照组骨缺损界限仍清晰。nBGC支架内大量新骨组织形成,而在CPC植入组新生骨只沉积于植入物的表面,CPC周围有厚纤维组织条带包裹。结论:nBGC支架在体内既能降解又具有生物活性,能融入骨重塑过程,具有良好的组织反应性和骨修复作用,是一种良好的组织工程骨支架材料。

Objective: To evaluate the tissue response to the implanting of nBGC and the effect of nBGC on bone repair. Methods: One 10mm-diameter cavernous bone defect was created in each tibia of the dogs. The nBGC scaffolds seeded with au-tologous osteoblasts were placed into one bone defect of each dog. The other bone defect was filled with self-setting calcium phosphate cement (CPC) as control. Dogs were terminated and specimens were procured at 8-week after surgery for X-ray, histology and teracycline immunofluoresence observation in order to compare the patterns of bone repair between two groups. Results: Radiographic union was established rapidly at the interface between the host bone and the scaffolds. In contrast, the clear cut edges of the cortex of the host bone were found in the control group. Tetracycline labeling demonstrated that newly formed woven bone had filled the pores of the nBGC scaffolds, while new bone was not found inside the CPC, but only on it's surface. The process of the nBGC scaffold degradation and bone substitution was reminiscent of bone remodeling. In contrast, the CPC were encompassed thick fibrous tissue. Conclusion: The nBGC composite is a bioactive and biodegradable material and can be incorporated into bone metabolism instead of being a permanent implant. In may be possible to exploit this material to elicit the healing of bone defects in humans by using osteogenic cells.