摘要
采用 ABC 免疫酶标技术,观察了大鼠脾悬液和原位树状突细胞的 S-100蛋白免疫反应性。结果表明,脾悬液标本中的淋巴树状突细胞(LDC)和脾石蜡切片标本中的交错突细胞(IDC)、滤泡树状突细胞(FDC)均为显示棕黄色反应的 S-100蛋白阳性细胞。结合扫描电镜和透射电镜观察的结果,支持了 LDC 在体内的等同细胞是 IDC 的观点。
With avid in-biotin complex immunoperoxidase technique,the immunoreactivities for S-100 protein of lymphoid dendritic cells(LDCs)in isolated LDC-enriched fraction and in situ of rat spleen were observed.The results showed that the immunoreaetivi(?)ies of LDCs in cell suspensions and follicalar dendritic cells of splenic corpuscles and interdi -gitating dendritic cells(IDCs)of periarterial lymphatic sheaths in paraffin sections were all strongly positive(brown-yellow in colour).Combined with the results from SEM and TEM,it supports the view that the counterparts of LDC in situ is IDC.
出处
《南京铁道医学院学报》
1992年第2期65-68,共4页
Journal of Nanjing Railway Medical College
基金
国家自然科学基金
江苏省科委资助
关键词
树状突细胞
免疫组织化学
脾
大鼠
dendritic cells
S-100 protein
immunohistochemistry
spleen
rats