甲状腺素水平改变对抗癫痫药物治疗的影响

The effect of varied thyroid hormones on antiepilepsy drug therapy

目的 探讨甲状腺素水平改变对抗癫痫药物(AEDs)治疗效果的影响。方法 采用免疫细胞化学染色法,将实验组Wistar大鼠分别予以苯巴比妥、苯巴比妥加甲状腺素、苯巴比妥加丙硫氧嘧啶等处理。处理4周后用马桑内酯(CL)诱发癫痫。2.5 h后,采血测定甲状腺激素指标,并测脑星形胶质细胞胶质原纤维酸性蛋白(GFAP)的表达。结果 苯巴比妥组和苯巴比妥加丙硫氧嘧啶组游离甲状腺素较对照组明显降低(P<0.01),促甲状腺激素显著增高(P<0.01),GFAP免疫反应显著减弱(P<0.01)。苯巴比妥加甲状腺素组GFAP免疫反应明显减弱(P<0.01)。结论 甲状腺素可使癫痫大鼠脑内GFAP表达抑制,甲状腺素水平升高可能增强苯巴比妥抗癫痫的效果。

Objective To study the effect of varied thyroid hormones on antiepilepsy drugs(AEDs). Methods There were 40 Wistar rats in our experiment which were divided into 4 groups: phenobarbital meal group; phenobarbital and thyroid hormones meal group;phenobarbital and propylthiouracil meal group;common meal group. After 4 weeks, the rats were induced to epilepsy by coriaria lactone,and were killed 2.5 h later to examine serum free triiodothyronine.free thyroxine, thyroid-stimulating hormone(TSH) and the expression degree of glial fibrillary acidic protein (GFAP) in astrocytes. Results Compared with the control group , phenobarbital meal group,phenobarbital and propylthiouracil meal group had lower free thyroid hormones (P<0.01),higher TSH (P<0. 01), weaker immunoreaction of GFAP (P<0. 01). The immunoreaction of GFAP was evidently weakened in the phenobarbital and thyroid hormones meal group (P< 0.01). Conclusions The thyroid hormones could depress the immunoreaction of GFAP in epilepsy rats. High level of thyroid hormones might improve the curative effectiveness on epilepsy by phenobarbital.