动物原代细胞彗星试验敏感性筛选

THE SCREENING OF SENSITIVE PRIMARY CELLS FOR THE COMET ASSAY

目的:筛选一种动物原代敏感细胞用于彗星试验,使该试验能更广泛地对环境样品的遗传毒性进行监测。方法:用重铬酸钾(K_2CrO_7)和过氧化氢(H_2O_2)作为受试物对小鼠的肝、脾、肾细胞进行彗星试验,观察K_2CrO_7和H_2O_2对这3种细胞造成的DNA损伤来判断细胞的敏感性。结果:以K_2CrO_7染毒的彗星试验中肝细胞的检测阈值(10nmol/L)低于为脾和肾(100nmol/L),以H_2O_2染毒的彗星试验中,在相同浓度下肝细胞迁移长度最长。结论:肝细胞具有敏感性高、自身活化能力强、取材方便、耗时短等优点,可以作为一种检测细胞应用于彗星试验对环境样品进行遗传毒性监测。

Objective: In order to select a kind of sensitive primary cells to be used specially in the comet assay to detect genotoxiciy of environmental samples. Method: The K2CrO7 and H2O2 were used as mutagen; The isolated liver, spleen and kidney cells from mice were exposed to different concentrations of K2CrO7 and H2O2. The DNA damage detected by comet assay was used to judge the sensitivity of the cells. Results: After exposure to K2CrO07, the detection threshold of liver cells( 10 nmol/L) was less than that of spleen and kidney cells(100nmol/L). After exposure to H2O2, the DNA migration length of liver cells was longest. Conclusion: The liver cells have the advantages of high sensitivity, auto - activation, convenience and quickness, so it can be selected as target cell of comet assay to detect genotoxiciy of environmental samples.