高效液相色谱法测定咽喉舒胶囊中甘草酸的含量

Determination of Glycyrrhizic acid in Yanhoushu capsule by RP-HPLC

目的 采用反相高效液相色谱法测定咽喉舒胶囊中甘草酸的含量。方法 以甲醇 1%醋酸 (6 4∶36 )为流动相 ,KromasilC18柱 (2 5 0mm× 4 .6mm ,5 μm)为固定相 ,流速为 1mL/min ,检测波长为 2 5 4nm。结果 甘草酸的测定在 0 .78～ 3.9μg/mL范围内线性关系良好 ,平均回收率为 10 1.1% ,RSD为 2 .2 8%。结论 方法简单、灵敏度高 。

OBJECTIVE To develop an RP HPLC method for the assay of Glycyrrhizic acid in Yanhoushu capsule. METHOD Separation was performed on a Kromasil C 18 (250mm×4.6mm,5μm) column. The mobile phase consists of CH 3OH:1%CH 3COOH(64∶36).The flow rate was 1mL/min. The UV detection was set at 254nm.RESULTS Glycyrrhizic acid had a good linear relation in the range of 0.78～3.9μg/mL, the average recovery was 101.1% and the RSD was 2.28%. CONCLUSION The method was simple, sensitive and rapid and it can be applied to the content determination of Yanhoushu Capsule.