大鼠脑胶质瘤伽玛刀治疗对细胞凋亡和Caspase-3、Fas表达的影响

Study of apoptosis and Caspase-3,Fas expression in rat glioma after treatment with gamma knife

目的 研究大鼠脑胶质瘤伽玛刀治疗后肿瘤细胞凋亡的时程变化和Caspase 3、Fas的表达。方法 立体定向法制作C6大鼠脑胶质瘤模型 6 0只 ,分为治疗组和对照组 ,治疗组于肿瘤接种后 14d行伽玛刀治疗 ,治疗后不同时间点行流式细胞学检查测定肿瘤细胞的凋亡率以及Caspase 3和Fas基因蛋白表达情况 ,分析凋亡率与两种蛋白的相关性。结果 与对照组相比 ,治疗组肿瘤细胞的凋亡率明显增高 (P <0 0 5 ) ,治疗后 4 8h肿瘤细胞凋亡率达高峰 ,然后逐渐下降 ;Caspase 3和Fas的表达情况与凋亡率的变化呈正相关 (r1 =0 92 8,r2 =0 916 )。结论 肿瘤细胞的凋亡为伽玛刀治疗胶质瘤疗效之一 ,Caspase 3和Fas基因可能参与和调节伽玛刀照射后肿瘤细胞凋亡。

Objective To investigate the apoptosis and Caspase-3,Fas expression in rat glioma after treatment with gamma knife. Methods Setting up C6 glioma model with 60 rats,which were devided into a treatment group( n =30) and a control group( n =30). On the 14 th day after planting glioma cells,rats of the treatment group were subjected to gamma knife irradiation.At the 12 th hr,24 th hr,48 th hr,7 th day,14 th day,21 st day,flow cytometry was performed to estimate the glioma cells apoptosis and the expression of Caspase-3 and Fas.The relation between apoptosis and the two kinds of proteins was analysed. Results Compared with the control group,the apoptosis rate of the glioma cells in the treatment group increased obviously ( P <0.05),and at the 48 th hr reached its peak,then decreased gradually.The expression of Caspase-3 and Fas was positively correlated with apoptosis( r 1=0.928, r 2=0.916). Conclusion The apoptosis of the tumor cells is a kind of effect of gamma knife treatment.Caspase-3 and Fas gene may take part in the regulation of apoptosis.