摘要
实验兔42只分成正常对照组、去甲肾上腺素1组(NE1)、NE2组,用药后分别于12h、24h、48h采血测定各组NE浓度,并处死取心肌做病理组织学检查,半定量分析心肌病理损害的程度。结果发现:NE引起兔心肌明显损害,细胞浸润及血栓形成等,随着NE剂量加大,心肌损害加重,死亡兔子数目增多。结论:NE心肌损害模型以NE1mg/kg,观察时间24h为宜。
Forty-two New Zealand rabbits were divided into three groups: control, norepinephrine (NE) -1 and NE-2. NE administered in NE- 1 and NE-2 was 1 mg /kg and 2 mg/kg respectively. Blood samples were taken 12 hr, 24 hr, and 48 hr after NE treatment and the serum level of NE was determined. Semi-quantitatiye histological scoring syslem was adopted to estimate the extent of myocardial damage. NE is found to cause significant myocardial injuries, including myocardial degeneration, necrosis with leucocytic infiltration, thrombus formation, etc. The. severity of the myocardial damage increases with the increment of the amount of NE administered. As dose of NE was increased the mortality rate of rabbits got higher accordingly. The authors assume that an intravenous dose cf NE 1 mg /kg body weight of rabbit is satisfactory for 24 hr experimental observation.
基金
国家自然科学基金资助项目之一部分
关键词
去甲肾上腺素
心肌损害
兔
Key words norepinephrine
myocardial damage
rabbits
