黄芪提取物的体外抗乙肝病毒作用

Anti-HBV activity of extracts from astragalus

目的 研究黄芪提取物抗乙型肝炎病毒 (HBV)作用。方法 采用ELISA法观察两种黄芪提取物黄芪总苷(AST)、黄芪多糖 (ASP)对HBV DNA转染的HepG2 2 2 15细胞分泌表面抗原 (HBsAg)和e抗原 (HBeAg)影响 ,同时用3 H TdR掺入法检测HepG2 2 2 15细胞增殖。结果 AST( 4 0、80、16 0mg/L)、ASP( 2 5 6、5 12mg/L)对HBsAg、HBeAg分泌有抑制作用 ,ASP( 12 8mg/L)还可以抑制HBsAg分泌。AST( 4 0、80、16 0mg/L)、ASP( 12 8、2 5 6、5 12mg/L)可抑制HepG2 2 2 15细胞的增殖。抑制HepG2 2 2 15细胞分泌HBeAg、HBsAg和细胞增殖的半数有效浓度 (EC50 ) ,AST为88 14、92 0 7、32 81mg/L ,ASP为 35 0 5 9、35 4 38、139 5 5mg/L。结论 AST、ASP在体外实验中有抗HBV和抑制HepG2 2 2 15细胞增殖的作用 ,AST作用优于ASP。

Objective To study the anti HBV activity of extracts from astragalus in vitro. Methods HepG 2 2 2 15 cell line was cultured with AST or ASP for 5 days. HBsAg and HBeAg were assayed by ELISA method and proliferation was measured by 3H TdR. Results The secretion of HBsAg and HBeAg of 2 2 15 cells were reduced significantly by AST(40,80,160 mg/L) and ASP(256, 512 mg/L); HBsAg was also significantly decreased by ASP (128 mg/L). The growth of HepG 2 2 2 15 cell was inhibited by AST(160,80,40 mg/L) and ASP(128,256,512 mg/L). The EC 50 of AST and ASP that inhibited HepG 2 2 2 15 cell’s secretion of HBeAg, HBsAg and cell proliferation was 88 14,92 07,32 81 mg/L and 350 59,354 38,139 55 mg/L respectively. Conclusion AST and ASP have the anti HBV activity in vitro and inhibit on the growth of HepG 2 2 2 15 cells.