高强度聚焦超声治疗VX2骨肿瘤的免疫变化

Immune changes in bone neoplasm rabbits transplanted with VX2 before and after high intensity focused ultrasound therapy

目的 探讨高强度聚焦超声 (HIFU )治疗骨肿瘤前后的免疫学变化。方法 将新西兰大白兔分为HIFU治疗组 (2 3只 )及正常对照组 (10只 ) ,对HIFU治疗组兔建立VX2移植性骨肿瘤模型 ,2周后接受HIFU治疗 ,于不同时间处死 ,免疫组织化学染色观察HSP70表达的变化 ,并在治疗前后抽血检测CD2 5变化。结果 治疗前肿瘤组织HSP70阳性表达细胞数量少 ,治疗后即刻直到 2 1d一直呈强阳性表达且阳性表达的细胞数明显增加。治疗前CD2 5为 7.83± 2 .0 3与正常兔 13 .18± 3 .47比较差异有显著性 (P <0 .0 5 ) ;治疗后增高为 15 .5 5± 2 .69,与治疗前比较差异有非常显著性 (P <0 .0 1)。结论 HIFU治疗后骨恶性肿瘤组织HSP70、CD2 5的数量增多 ,可递呈特异性抗原给T细胞 ,刺激T细胞增殖 。

Objective To study the immune changes before and after high intensity focused ultrasound (HIFU) therapy.Methods Thirty-three rabbits were divided into 2 groups: HIFU group (n=23),control group (n=10).Twenty-three bone neoplasm rabbits transplanted with VX2 for 2 weeks were subjected to HIFU therapy,and killed at different time points (immediately,1 day,3 day,7 day,14 day,21 day after therapy).The HSP70 changes were observed by immunohistochemical examination.The changes of CD25 were also examined before and 2 weeks after HIFU therapy by using flow cytometry.Results CD25 was significantly increased after treatment as compared with that before treatment.From day 0 to day 21 after therapy,the expression of HSP70 was positive and stronger than that before therapy.Conclusion HIFU therapy could enhance HSP70 and CD25 expression in bone neoplasm,which may present the antigens to T cell and stimulate host immune system,as well as improve the anti-tumor immune activity.