粘着斑激酶在乳腺癌组织中表达的研究

Immunohistochemical study on expression of FAK in human breast carcinoma

目的 研究粘着斑激酶 (FAK)表达与乳腺癌生物学行为和预后的关系。方法 应用免疫组织化学 S-P方法检测 1 1 5例乳腺癌手术标本中 FAK的表达情况。结果 乳腺癌原发灶 FAK表达阳性率为 81 .74 % ,其中无区域淋巴结转移者阳性率为 6 4 .4 4 % ,有区域淋巴结转移者阳性率为 92 .86 % (P<0 .0 1 ) ,FAK表达水平与乳腺癌大小和 p TNM分期进展呈正相关 (P<0 .0 1 ,P<0 .0 1 )。FAK阳性者术后 3年、 5年生存率分别为 6 5 .96 %、 4 5 .39% ,而阴性者为 76 .1 9%、 6 0 .6 1 % ;两组患者术后 3年、 5年生存率差别均有显著性 (P<0 .0 5 ,P<0 .0 5 )。结论 乳腺癌组织由于 FAK表达增加 ,导致恶性细胞的发生、增殖、浸润和转移。故检测乳腺癌组织中 FAK表达状况 。

Objective To study the relation between FAK expression and biological behavior or prognosis in breast carcinoma.Method The expression of FAK was examined immunohistochemically in resected breast carcinoma,using FAK anti monoclonal antibody.Results Of 115 patients tested,81 74% showed positive staining of FAK in the primary lesions,while others were negative.Tumors without regional nodal metastasis showed significantly lower positive staining rate (64 44%) than those with nodal involvement(92 86%,P<0 01).Expression of FAK is correlated with the size and the progression of pTNM stage (P<0 01).survival rate at 3 and 5 years was 65 96% and 45 39% respectively for positive staining group of FAK,compared to 76 19% and 60 61% respectively for negative staining group(P<0 01).Conclusions The data indicated that FAK increased proliferation,invasion and metastasis.The expression of FAK may be a useful tool for prognosis of human breast carcinoma.