一阶导数光谱法测定枯痔注射液中盐酸普鲁卡因的含量

Determination of Procaine in Kuzhi Injection by First Derivative Spectrophotometry

目的 测定枯痔注射液中盐酸普鲁卡因的含量。方法 一阶导数分光光度法。结果 盐酸普鲁卡因的峰和谷分别为 2 30nm和 2 6 0nm ,在 5 .0～ 2 0 .0 μg·ml-1范围内D值与浓度呈良好线性关系 (r =0 .9983)。回收率为 99.7% ,RSD为 0 .6 %(n =5 )。结论 此方法快速、准确、重现性好 ,可做为枯痔注射液的含量测定方法。

Aim To establish a method for the determination of the content of Procaine in Ku Zhi injection.Methods First derivative spectrophotometry was used.Results There was sbsorption peak and trough lf simvastation at 230nm and 260nm.A Linear correlation was obtained over the range of 5.0～20.0μg·ml -1 (r=0.998 3) of procaine.The average recovevy of method was 99.7%,RSD=0.6%(n=5).Conclusion The method was proved to be simple,accurate and reproducible.