EBV反义LMP-1转染CNE2细胞对裸鼠致瘤的作用

Antisense LMP1 Gene Reduced the Tumorgenicity of CNE2 Cells in the Nude Mice

目的研究反义LMP1基因转染CNE2细胞对裸鼠的成瘤作用。方法用反义LMP1转染的CNE2细胞、空载体转染的CNE2细胞以及原始CNE2细胞分别接种裸鼠,观察三种细胞的成瘤能力及细胞的恶性程度。结果反义LMP1转染的CNE2细胞比空载体转染的CNE2细胞及原始CNE2细胞在裸鼠体内成瘤能力(包括平均瘤重及总瘤重)和镜下细胞恶性程度都有所降低。结论反义LMP1能成功抑制LMP1的表达,逆转NPC细胞的恶性表型,为临床应用反义技术进行鼻咽癌基因治疗提供了实验依据。

Objective To study the tumorgenicity of the CNE2 cell transferred with the antisense LMP1 gene. Methods Nude mice were injected with CNE2 cell transferred with the antisense LMP1 gene, CNE2 cell transferred vector, or CNE2 cell. The formations of tumors in the injected nude mice were observed. Results The tumorgenicity of the CNE2 cell transferred with antisense LMP1 gene was lower than other two cell lines. Conclusion The antisense LMP1 gene can inhibit EBV LMP1 expression and inhibit the growth of NPC cells in the nude mice. This study provided experimental basis for the clinical gene therapy of NPC with antisense gene technique.