丰宫并殖吸虫实验宿主感染的研究

Study of the Infected Hosts of Paragonimus proliferus

目的进一步研究丰宫并殖吸虫的囊蚴及其宿主性。方法从丰宫并殖吸虫流行区采集溪蟹,鉴定种类,分离囊蚴及后尾蚴,观测后感染实验宿主,收集成虫制片观测鉴定。结果绝大多数后尾蚴在蟹体内不形成囊壁,形成囊壁的囊蚴体积巨大在1mm以上,一层极薄易碎的囊壁。第二中间宿主为景洪溪蟹和毛足溪蟹,前者的自然感染率为53.19%,后者为66.67%。丰宫并殖吸虫的适宜实验终宿主是大白鼠,犬、猫为不适宜宿主。结论宫并殖吸虫囊蚴的大小在1mm以上,是已知的并殖吸虫囊蚴中之最大者。第二中间宿主为景洪溪蟹和毛足溪蟹。大白鼠是其适宜的实验终宿主。

Objective Investigation of the metacercaria and hosts of Paragonimus proliferus. Methods Crabs were collected and classified from the epidemic area of P.proliferus. Metacercaria and excysted metacercaria were collected and used to infect experimental hosts. Adult worms were collected and analyzed. The infected hosts were under detail observation. Results Most of them did not form the metacercaria in crabs. Only few metacercaria were obtained. The metacercaria is larger than 1mm and is wrapped with one thin and fragile cyst wall. The second intermediate hosts are Potamon chinghungense and Potamon hispidum, with natural infectious rates of 53.19% and 66.67% respectively. Rat is a suitable host for for P.proliferus but not dogs or cats. Conclusions The size of metacercaria of P.proliferus is larger than 1mm and is the largest of all known species. The second intermediate hosts are P.chinghungense and P.hispidum, and the proper experimental definitive host is rats.